Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit | NNMT elisa kit

Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit

Cat. Num. AAA453001

• Reactivity: Human
• Synonyms: Nicotinamide-N-Methyltransferase (NNMT); Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit; NNMT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NNMT. No significant cross-reactivity or interference between NNMT and analogues was observed.
• Sequence Length: 264

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NNMT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NNMT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NNMT elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NNMT in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NNMT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NNMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NNMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the naddition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NNMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5453790
• NCBI GeneID: 4837
• NCBI Accession #: NP_006160.1
• NCBI GenBank Nucleotide #: NM_006169.2
• UniProt Accession #: P40261
• Molecular Weight: 29,574 Da
• NCBI Official Full Name: nicotinamide N-methyltransferase
• NCBI Official Synonym Full Names: nicotinamide N-methyltransferase
• NCBI Official Symbol: NNMT
• NCBI Protein Information: nicotinamide N-methyltransferase
• UniProt Protein Name: Nicotinamide N-methyltransferase
• UniProt Gene Name: NNMT

NCBI Description

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

Uniprot Description

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.1.1.1; Methyltransferase

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function: pyridine N-methyltransferase activity

Biological Process: methylation