Human NF-kappa-B inhibitor delta (NFKBID) ELISA Kit | NFKBID elisa kit

Human NF-kappa-B inhibitor delta (NFKBID) ELISA Kit

Cat. Num. AAA756385

• Gene Names: NFKBID; TA-NFKBH; IkappaBNS
• Reactivity: Human
• Synonyms: NF-kappa-B inhibitor delta (NFKBID); Human NF-kappa-B inhibitor delta (NFKBID) ELISA Kit; NFKBID elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500ng/mL
• Sensitivity: 1.0 ng/mL
• Intended Uses: This N1-N12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human N1-N12. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFKBID elisa kit

Principle of the assay: N1-N12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-N1-N12 antibody and an N1-N12-HRP conjugate. The assay sample and buffer are incubated together with N1-N12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the N1-N12 concentration since N1-N12 from samples and N1-N12-HRP conjugate compete for the anti-N1-N12 antibody binding site. Since the number of sites is limited, as more sites are occupied by N1-N12 from the sample, fewer sites are left to bind N1-N12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The N1-N12 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 41281709
• NCBI GeneID: 84807
• NCBI Accession #: NP_640332.1
• NCBI GenBank Nucleotide #: NM_139239.1
• Molecular Weight: 15,921 Da
• NCBI Official Full Name: NF-kappa-B inhibitor delta
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, delta
• NCBI Official Symbol: NFKBID
• NCBI Official Synonym Symbols: TA-NFKBH; IkappaBNS
• NCBI Protein Information: NF-kappa-B inhibitor delta; ikB-delta; ikappaBdelta; I-kappa-B-delta; T-cell activation NFKB-like protein
• UniProt Protein Name: NF-kappa-B inhibitor delta
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBID
• UniProt Synonym Gene Names: IKBNS; IkB-delta; IkappaBdelta
• UniProt Entry Name: IKBD_HUMAN

Uniprot Description

NFKBID: May regulate the expression of IL-2, IL-6, and other cytokines through regulation on NF-kappa-B activity. Functions in the regulation of inflammatory responses. May also regulate TCR- induced negative selection of thymocytes. Belongs to the NF-kappa-B inhibitor family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: nucleus

Molecular Function: NF-kappaB binding

Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of T cell differentiation in the thymus; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response