Typical Testing Data/Standard Curve (for reference only)
Human Neuropeptide S Receptor (NPSR) ELISA Kit | NPSR elisa kit
Human Neuropeptide S Receptor (NPSR) ELISA Kit
Gene Names
NPSR1; GPRA; NPSR; VRR1; ASRT2; PGR14; GPR154
Reactivity
Human
Synonyms
Neuropeptide S Receptor (NPSR); Human Neuropeptide S Receptor (NPSR) ELISA Kit; ASRT2; GPR154; GPRA; NPSR1; PGR14; VRR1; G Protein-Coupled Receptor For Asthma Susceptibility; G Protein-Coupled Receptor 154; G-protein coupled receptor PGR14; NPSR elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NPSR. No significant cross-reactivity or interference between NPSR and analogues was observed.
Sequence Length
366
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.108ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPSR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPSR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NPSR elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NPSR in human tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPSR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NPSR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NPSR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NPSR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPSR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NPSR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NPSR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NPSR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,612 Da
NCBI Official Full Name
neuropeptide S receptor B short
NCBI Official Synonym Full Names
neuropeptide S receptor 1
NCBI Official Symbol
NPSR1
NCBI Official Synonym Symbols
GPRA; NPSR; VRR1; ASRT2; PGR14; GPR154
NCBI Protein Information
neuropeptide S receptor
UniProt Protein Name
Neuropeptide S receptor
UniProt Gene Name
NPSR1
UniProt Synonym Gene Names
GPR154; GPRA; PGR14
NCBI Description
This gene encodes a member of the vasopressin/oxytocin subfamily of G protein-coupled receptors. The encoded membrane protein acts as a receptor for neuropeptide S and affects a variety of cellular processes through its signaling. Increased expression of this gene in ciliated cells of the respiratory epithelium and in bronchial smooth muscle cells is associated with asthma. Polymorphisms in this gene have also been associated with asthma susceptibility, panic disorders, inflammatory bowel disease, and rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
G-protein coupled receptor for neuropeptide S (NPS) (PubMed:16790440). Promotes mobilization of intracellular Ca2+ stores (PubMed:16790440). Inhibits cell growth in response to NPS binding (PubMed:15947423). Involved in pathogenesis of asthma and other IgE-mediated diseases.
Research Articles on NPSR
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Product Notes
The Human NPSR npsr1 (Catalog #AAA450104) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA450104 ELISA Kit recognizes Human NPSR. It is sometimes possible for the material contained within the vial of "Neuropeptide S Receptor (NPSR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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