Human Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit | NAIP elisa kit

Human Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit

Cat. Num. AAA2019676

• Gene Names: NAIP; BIRC1; NLRB1; psiNAIP
• Reactivity: Human
• Synonyms: Neuronal Apoptosis Inhibitory Protein (NAIP); Human Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit; BIRC1; NLRB1; PsiNAIP; NLR Family; Apoptosis Inhibitory Protein; Baculoviral IAP Repeat-Containing Protein 1; NAIP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuronal Apoptosis Inhibitory Protein (NAIP).; No significant cross-reactivity or interference between Neuronal Apoptosis Inhibitory Protein (NAIP) and analogues was observed.
• Sequence Length: 1403

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuronal Apoptosis Inhibitory Protein (NAIP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuronal Apoptosis Inhibitory Protein (NAIP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NAIP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal Apoptosis Inhibitory Protein (NAIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuronal Apoptosis Inhibitory Protein (NAIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuronal Apoptosis Inhibitory Protein (NAIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuronal Apoptosis Inhibitory Protein (NAIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NAIP elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 2642133
• NCBI GeneID: 4671
• UniProt Accession #: Q13075; O75857; Q13730; Q59GI6; Q8TDZ4; Q99796; B9EG72; E9PHD1
• Molecular Weight: 141,329 Da
• NCBI Official Full Name: neuronal apoptosis inhibitory protein
• NCBI Official Synonym Full Names: NLR family, apoptosis inhibitory protein
• NCBI Official Symbol: NAIP
• NCBI Official Synonym Symbols: BIRC1; NLRB1; psiNAIP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 1
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 1
• Protein Family: Putative niacin/nicotinamide transporter
• UniProt Gene Name: NAIP
• UniProt Synonym Gene Names: BIRC1
• UniProt Entry Name: BIRC1_HUMAN

NCBI Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that this gene is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. The protein encoded by this gene contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BIRC1: Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor- neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: spindle microtubule; basolateral plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ATP binding

Biological Process: nervous system development; regulation of signal transduction; protein ubiquitination; innate immune response; negative regulation of neuron apoptosis; negative regulation of caspase activity; inflammatory response; negative regulation of apoptosis