Porcine Neuronal apoptosis inhibitory protein ELISA Kit | NAIP elisa kit

Porcine Neuronal apoptosis inhibitory protein ELISA Kit

Cat. Num. AAA752865

• Gene Names: NAIP; BIRC1; NLRB1; psiNAIP
• Reactivity: Porcine
• Synonyms: Neuronal apoptosis inhibitory protein; Porcine Neuronal apoptosis inhibitory protein ELISA Kit; NAIP elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This NAIP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine NAIP. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This NAIP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine NAIP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NAIP elisa kit

Principle of the Assay: NAIP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NAIP antibody and an NAIP-HRP conjugate. The assay sample and buffer are incubated together with NAIP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NAIP concentration since NAIP from samples and NAIP-HRP conjugate compete for the anti-NAIP antibody binding site. Since the number of sites is limited, as more sites are occupied by NAIP from the sample, fewer sites are left to bind NAIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAIP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NAIP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 2642133
• NCBI GeneID: 4671
• Molecular Weight: 141,329 Da
• NCBI Official Full Name: neuronal apoptosis inhibitory protein
• NCBI Official Synonym Full Names: NLR family, apoptosis inhibitory protein
• NCBI Official Symbol: NAIP
• NCBI Official Synonym Symbols: BIRC1; NLRB1; psiNAIP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 1; neuronal apoptosis inhibitory protein; psi neuronal apoptosis inhibitory protein; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domain containing 1
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 1
• Protein Family: Putative niacin/nicotinamide transporter
• UniProt Gene Name: NAIP
• UniProt Synonym Gene Names: BIRC1
• UniProt Entry Name: BIRC1_HUMAN

NCBI Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that this gene is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. The protein encoded by this gene contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BIRC1: Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor- neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: basolateral plasma membrane; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ATP binding

Biological Process: nervous system development; regulation of signal transduction; innate immune response; protein ubiquitination; negative regulation of caspase activity; negative regulation of neuron apoptosis; inflammatory response; negative regulation of apoptosis