Human Neuromedin B (NMB) ELISA Kit | NMB elisa kit

Human Neuromedin B (NMB) ELISA Kit

Cat. Num. AAA2707496

• Reactivity: Human
• Synonyms: Neuromedin B (NMB); Human Neuromedin B (NMB) ELISA Kit; NM-B; NMB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuromedin B (NMB).; No significant cross-reactivity or interference between Neuromedin B (NMB) and analogues was observed.
• Sequence Length: 121

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.054ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuromedin B (NMB) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuromedin B (NMB) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NMB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuromedin B (NMB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuromedin B (NMB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuromedin B (NMB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuromedin B (NMB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45505143
• NCBI GeneID: 4828
• NCBI Accession #: NP_066563.2
• NCBI GenBank Nucleotide #: NP_066563.2
• UniProt Accession #: P08949
• NCBI Official Full Name: neuromedin-B isoform 1 preproprotein
• NCBI Official Synonym Full Names: neuromedin B
• NCBI Official Symbol: NMB
• NCBI Protein Information: neuromedin-B
• UniProt Protein Name: Neuromedin-B
• Protein Family: Neuromedin
• UniProt Gene Name: NMB
• UniProt Entry Name: NMB_HUMAN

NCBI Description

This gene encodes a member of the bombesin-like family of neuropeptides, which negatively regulate eating behavior. The encoded protein may regulate colonic smooth muscle contraction through binding to its cognate receptor, the neuromedin B receptor (NMBR). Polymorphisms of this gene may be associated with hunger, weight gain and obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

NMB: Stimulates smooth muscle contraction in a manner similar to that of bombesin. Belongs to the bombesin/neuromedin-B/ranatensin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q22-qter

Cellular Component: neuron projection; extracellular region

Molecular Function: hormone activity; neuromedin B receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; cell-cell signaling; neuropeptide signaling pathway; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of hormone secretion; glucose homeostasis; signal transduction; arachidonic acid secretion