Human coagulation factor X, FX ELISA Kit | FX elisa kit

Human coagulation factor X, FX ELISA Kit

Cat. Num. AAA161653

• Gene Names: F10; FX; FXA
• Reactivity: Human
• Synonyms: coagulation factor X; FX; Human coagulation factor X; FX ELISA Kit; FX elisa kit

• Reactivity: Human
• Sequence Length: 488

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.5ng/ml - 100ng/ml
• Sensitivity: 0.26ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FX elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Coagulation factor X (also known as FX) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human FX antibody. FX present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human FX Antibody is added and binds to FX in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FX antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human FX. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 182390
• NCBI GeneID: 2159
• UniProt Accession #: P00742; Q14340
• Molecular Weight: 54,732 Da
• NCBI Official Full Name: coagulation factor X
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X
• UniProt Protein Name: Coagulation factor X
• UniProt Gene Name: F10

NCBI Description

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

Uniprot Description

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.