Rabbit anti-Human TBP Polyclonal Antibody | anti-TBP antibody

TBP, CT (TATA-box-binding Protein, TATA-box Factor, TATA-binding Factor, TATA Sequence-binding Protein, Transcription Initiation Factor TFIID TBP Subunit, TFIID, TF2D, GTF2D1) (PE)

Cat. Num. AAA6502029

• Gene Names: TBP; HDL4; GTF2D; SCA17; TFIID; GTF2D1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TBP; Polyclonal Antibody; CT (TATA-box-binding Protein; TATA-box Factor; TATA-binding Factor; TATA Sequence-binding Protein; Transcription Initiation Factor TFIID TBP Subunit; TFIID; TF2D; GTF2D1) (PE); anti-TBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TBP.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TBP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human TBP (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TBP antibody (C-term) in HL60 cell line lysates (35ug/lane). TBP (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TBP Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-TBP antibody

Antibodies; Transcription Factors

References

1. Ohi, K., Genes Brain Behav. 8(4), 473-480 (2009). 2. Reid, S.J., Brain Res. 1268, 190-199 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507379
• NCBI GeneID: 6908
• NCBI Accession #: NP_003185
• NCBI GenBank Nucleotide #: NM_003194.4
• UniProt Accession #: P20226; Q16845; Q6IBM6; Q9UC02; B4E3B3; F5H869
• Molecular Weight: 37,698 Da
• NCBI Official Full Name: TATA-box-binding protein isoform 1
• NCBI Official Synonym Full Names: TATA box binding protein
• NCBI Official Symbol: TBP
• NCBI Official Synonym Symbols: HDL4; GTF2D; SCA17; TFIID; GTF2D1
• NCBI Protein Information: TATA-box-binding protein; TATA-box factor; TATA sequence-binding protein; TATA-box binding protein N-terminal domain; transcription initiation factor TFIID TBP subunit
• UniProt Protein Name: TATA-box-binding protein
• Protein Family: TBP-like protein
• UniProt Gene Name: TBP
• UniProt Synonym Gene Names: GTF2D1; TF2D; TFIID
• UniProt Entry Name: TBP_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

TBP: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with UTF1. Interacts with BRF2. Interacts with UBTF. Interacts with GPBP1. Interacts with CITED2. Interacts with ATF7IP. Interacts with HIV-1 Tat. Widely expressed, with levels highest in the testis and ovary. Belongs to the TBP family.

Protein type: Transcription initiation complex; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: nucleoplasm; transcription factor TFIID complex; male pronucleus; cytoplasm; female pronucleus; transcription factor TFIIA complex

Molecular Function: protein binding; transcription factor binding; transcription factor activity

Biological Process: chromatin silencing at rDNA; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase II promoter; gene expression; spermatogenesis; transcription initiation from RNA polymerase I promoter

Disease: Parkinson Disease, Late-onset; Spinocerebellar Ataxia 17