Human Neuroepithelial Cell Transforming Gene 1 (NET1) ELISA Kit | NET1 elisa kit

Human Neuroepithelial Cell Transforming Gene 1 (NET1) ELISA Kit

Cat. Num. AAA453801

• Gene Names: NET1; NET1A; ARHGEF8
• Reactivity: Human
• Synonyms: Neuroepithelial Cell Transforming Gene 1 (NET1); Human Neuroepithelial Cell Transforming Gene 1 (NET1) ELISA Kit; ARHGEF8; NET1A; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8; NET1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NET1. No significant cross-reactivity or interference between NET1 and analogues was observed.
• Sequence Length: 596

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.125ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NET1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NET1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NET1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NET1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NET1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NET1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NET1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NET1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 114145479
• NCBI GeneID: 10276
• NCBI Accession #: NP_001040625.1
• NCBI GenBank Nucleotide #: NM_001047160.2
• UniProt Accession #: Q7Z628; Q12773; Q96D82; Q99903; Q9UEN6
• Molecular Weight: 61,885 Da
• NCBI Official Full Name: neuroepithelial cell-transforming gene 1 protein isoform 1
• NCBI Official Synonym Full Names: neuroepithelial cell transforming 1
• NCBI Official Symbol: NET1
• NCBI Official Synonym Symbols: NET1A; ARHGEF8
• NCBI Protein Information: neuroepithelial cell-transforming gene 1 protein
• UniProt Protein Name: Neuroepithelial cell-transforming gene 1 protein
• Protein Family: Nucleolar protein
• UniProt Gene Name: NET1
• UniProt Synonym Gene Names: ARHGEF8

NCBI Description

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.