Human Netrin receptor UNC5C ELISA Kit | UNC5C elisa kit

Human Netrin receptor UNC5C ELISA Kit

Cat. Num. AAA761169

• Reactivity: Human
• Synonyms: Netrin receptor UNC5C; Human Netrin receptor UNC5C ELISA Kit; UNC5C/UNC5H3/netrin receptor UNC5C/Protein unc-5 homolog 3/Protein unc-5 homolog C/unc-5 homolog 3/unc-5 homolog C (C. elegans)/UNC5H3unc5 (C.elegans homolog) c; UNC5C elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of UMOD. No significant cross-reactivity or interference between UMOD and analogues was observed.
• Sequence Length: 931

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UMOD were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UMOD were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UNC5C elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-UMOD antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-UMOD antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the UMOD amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of UMOD can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 40786503
• NCBI GeneID: 362049
• NCBI Accession #: NP_955439.1
• NCBI GenBank Nucleotide #: NM_199407.1
• Molecular Weight: 103,135 Da
• NCBI Official Full Name: netrin receptor UNC5C
• NCBI Official Synonym Full Names: unc-5 netrin receptor C
• NCBI Official Symbol: Unc5c
• NCBI Protein Information: netrin receptor UNC5C
• UniProt Protein Name: Netrin receptor UNC5C
• Protein Family: Netrin receptor
• UniProt Gene Name: Unc5c
• UniProt Entry Name: UNC5C_RAT

NCBI Description

member of the UNC-5 family of netrin receptors; may be involved in cerebellar development [RGD, Feb 2006]

Uniprot Description

UNC5C: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Belongs to the unc-5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: cell junction; integral to membrane; neuron projection; plasma membrane; synapse

Molecular Function: netrin receptor activity

Biological Process: anterior/posterior axon guidance; apoptosis; axon guidance; brain development; multicellular organismal development; positive regulation of apoptosis; regulation of cell migration; signal transduction