Human Natural Cytotoxicity Triggering Receptor 3 (NCR3) ELISA Kit | NCR3 elisa kit

Human Natural Cytotoxicity Triggering Receptor 3 (NCR3) ELISA Kit

Cat. Num. AAA2707552

• Gene Names: NCR3; 1C7; MALS; CD337; LY117; NKp30
• Reactivity: Human
• Synonyms: Natural Cytotoxicity Triggering Receptor 3 (NCR3); Human Natural Cytotoxicity Triggering Receptor 3 (NCR3) ELISA Kit; CD337; 1C7; LY117; NKp30; Lymphocyte Antigen 117; Activating natural killer receptor p30; Natural killer cell p30-related protein; NCR3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Natural Cytotoxicity Triggering Receptor 3 (NCR3).; No significant cross-reactivity or interference between Natural Cytotoxicity Triggering Receptor 3 (NCR3) and analogues was observed.
• Sequence Length: 177

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 6.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Natural Cytotoxicity Triggering Receptor 3 (NCR3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Natural Cytotoxicity Triggering Receptor 3 (NCR3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NCR3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Natural Cytotoxicity Triggering Receptor 3 (NCR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Natural Cytotoxicity Triggering Receptor 3 (NCR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Natural Cytotoxicity Triggering Receptor 3 (NCR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Natural Cytotoxicity Triggering Receptor 3 (NCR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224586860
• NCBI GeneID: 259197
• NCBI Accession #: NP_001138938.1
• NCBI GenBank Nucleotide #: NP_001138938.1
• UniProt Accession #: O14931
• NCBI Official Full Name: natural cytotoxicity triggering receptor 3 isoform b
• NCBI Official Synonym Full Names: natural cytotoxicity triggering receptor 3
• NCBI Official Symbol: NCR3
• NCBI Official Synonym Symbols: 1C7; MALS; CD337; LY117; NKp30
• NCBI Protein Information: natural cytotoxicity triggering receptor 3
• UniProt Protein Name: Natural cytotoxicity triggering receptor 3
• Protein Family: Natural cytotoxicity triggering receptor
• UniProt Gene Name: NCR3
• UniProt Synonym Gene Names: 1C7; LY117; NK-p30; NKp30
• UniProt Entry Name: NCTR3_HUMAN

NCBI Description

The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Uniprot Description

NCR3: Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation. Belongs to the natural cytotoxicity receptor (NCR) family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to plasma membrane

Biological Process: immune response; cell recognition; inflammatory response; positive regulation of natural killer cell mediated cytotoxicity

Disease: Malaria, Mild, Susceptibility To