Human NADH ubiquinone oxidoreductase chain 4L (MT ND4L) ELISA Kit | MT ND4L elisa kit

Human NADH ubiquinone oxidoreductase chain 4L (MT ND4L) ELISA Kit

Cat. Num. AAA7225312

• Gene Names: MT-ND4L; MTND4L; ND4L
• Reactivity: Human
• Synonyms: NADH ubiquinone oxidoreductase chain 4L (MT ND4L); Human NADH ubiquinone oxidoreductase chain 4L (MT ND4L) ELISA Kit; MT ND4L elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive or Sandwich
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MT ND4L elisa kit

Intended Uses: This MT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MT. This ELISA kit for research use only!

Principle of the Assay: MT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MT antibody and an MT-HRP conjugate. The assay sample and buffer are incubated together with MT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT concentration since MT from samples and MT-HRP conjugate compete for the anti-MT antibody binding site. Since the number of sites is limited, as more sites are occupied by MT from the sample, fewer sites are left to bind MT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 128888
• NCBI GeneID: 4539
• Molecular Weight: 10,741 Da
• NCBI Official Full Name: NADH-ubiquinone oxidoreductase chain 4L
• NCBI Official Synonym Full Names: mitochondrially encoded NADH 4L dehydrogenase
• NCBI Official Symbol: MT-ND4L
• NCBI Official Synonym Symbols: MTND4L; ND4L
• NCBI Protein Information: NADH dehydrogenase, subunit 4L (complex I); NADH dehydrogenase subunit 4L
• UniProt Protein Name: NADH-ubiquinone oxidoreductase chain 4L
• UniProt Gene Name: MT-ND4L
• UniProt Synonym Gene Names: MTND4L; NADH4L; ND4L
• UniProt Entry Name: NU4LM_HUMAN

Uniprot Description

MT-ND4L: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in MT-ND4L are a cause of Leber hereditary optic neuropathy (LHON). LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Belongs to the complex I subunit 4L family.

Protein type: Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; Mitochondrial; Oxidoreductase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: -

Disease: Leber Optic Atrophy