Typical Testing Data/Standard Curve (for reference only)
Human N acetylneuraminate lyase (NPL) ELISA Kit | NPL elisa kit
Human N acetylneuraminate lyase (NPL) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: NPL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NPL antibody and an NPL-HRP conjugate. The assay sample and buffer are incubated together with NPL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPL concentration since NPL from samples and NPL-HRP conjugate compete for the anti-NPL antibody binding site. Since the number of sites is limited, as more sites are occupied by NPL from the sample, fewer sites are left to bind NPL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPL concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
Uniprot Description
NPL: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Belongs to the DHDPS family. NanA subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 4.1.3.3; Lyase
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: cytosol
Molecular Function: N-acetylneuraminate lyase activity
Biological Process: N-acetylneuraminate catabolic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on NPL
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Product Notes
The Human NPL npl (Catalog #AAA7240436) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7240436 ELISA Kit recognizes Human NPL. It is sometimes possible for the material contained within the vial of "N acetylneuraminate lyase (NPL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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