Typical Testing Data/Standard Curve (for reference only)
Human Myosin VIIa (MYO7A) ELISA Kit | MYO7A elisa kit
Human Myosin VIIa (MYO7A) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MYO7A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MYO7A antibody and an MYO7A-HRP conjugate. The assay sample and buffer are incubated together with MYO7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYO7A concentration since MYO7A from samples and MYO7A-HRP conjugate compete for the anti-MYO7A antibody binding site. Since the number of sites is limited, as more sites are occupied by MYO7A from the sample, fewer sites are left to bind MYO7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYO7A concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
MYO7A: an actin-based motor molecule with ATPase activity and a calcium sensitive calmodulin binding subunit. May play a role in trafficking of ribbon- synaptic vesicle complexes and renewal of outer photoreceptor disks. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Seven alternatively spliced isoforms have been described.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 11q13.5
Cellular Component: stereocilium; photoreceptor outer segment; photoreceptor inner segment; lysosomal membrane; cytoplasm; apical plasma membrane; melanosome; synapse; cell cortex; cytosol; photoreceptor connecting cilium
Molecular Function: calmodulin binding; microfilament motor activity; actin filament binding; protein domain specific binding; protein binding; protein homodimerization activity; spectrin binding; ADP binding; actin-dependent ATPase activity; ATP binding
Biological Process: phagolysosome formation; intracellular protein transport; eye photoreceptor cell development; pigment granule transport; sensory perception of sound; visual perception; metabolic process; actin filament-based movement; lysosome organization and biogenesis; sensory perception of light stimulus; auditory receptor cell stereocilium organization and biogenesis; equilibrioception; post-embryonic organ morphogenesis
Disease: Deafness, Autosomal Dominant 11; Deafness, Autosomal Recessive 2; Usher Syndrome, Type I
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Product Notes
The Human MYO7A myo7a (Catalog #AAA7214347) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214347 ELISA Kit recognizes Human MYO7A. It is sometimes possible for the material contained within the vial of "Myosin VIIa (MYO7A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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