Human Myoglobin (MYO) ELISA Kit | MYO elisa kit

Human Myoglobin (MYO) ELISA Kit

Cat. Num. AAA268195

• Gene Names: MB; PVALB; myoglobgin
• Reactivity: Human
• Synonyms: Myoglobin (MYO); Human Myoglobin (MYO) ELISA Kit; MYO elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 154

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MYO concentration
• Assay Type: Sandwich
• Detection Range: 50 ng/ml-0.78 ng/ml
• Sensitivity: 0.1 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYO elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MYO monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 44955888
• NCBI GeneID: 4151
• NCBI Accession #: NP_976312.1
• NCBI GenBank Nucleotide #: NM_203378.1
• Molecular Weight: 17,184 Da
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB; myoglobgin
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

NCBI Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; brown fat cell differentiation; response to hypoxia; response to hormone stimulus; enucleate erythrocyte differentiation