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Typical Testing Data/Standard Curve (for reference only) ()

Human Myoferlin, MYOF ELISA Kit | MYOF elisa kit

Human Myoferlin, MYOF ELISA Kit

Reactivity
Human
Synonyms
Myoferlin; MYOF; Human Myoferlin; MYOF ELISA Kit; MYOF elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
2048
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
50-10000ng/L
Sensitivity
23.43ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for MYOF elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Myoferlin (also known as MYOF) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MYOF antibody. MYOF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MYOF Antibody is added and binds to MYOF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYOF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MYOF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myoferlin isoform 2
UniProt Protein Name
Myoferlin
Protein Family
UniProt Gene Name
Myof
UniProt Synonym Gene Names
Fer1l3; Kiaa1207

Uniprot Description

myoferlin: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. Belongs to the ferlin family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19|19 C2

Cellular Component: caveola; cytoplasmic vesicle; extracellular exosome; integral component of membrane; intracellular membrane-bound organelle; membrane; nucleus; plasma membrane

Molecular Function: phospholipid binding

Biological Process: cellular response to heat; myoblast fusion; plasma membrane repair; regulation of vascular endothelial growth factor receptor signaling pathway

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Product Notes

The Human MYOF myof (Catalog #AAA1606606) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1606606 ELISA Kit recognizes Human MYOF. It is sometimes possible for the material contained within the vial of "Myoferlin, MYOF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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