Human Multimerin 1 (MMRN1) ELISA Kit | MMRN1 elisa kit

Human Multimerin 1 (MMRN1) ELISA Kit

Cat. Num. AAA2019807

• Gene Names: MMRN1; ECM; MMRN; GPIa*; EMILIN4
• Reactivity: Human
• Synonyms: Multimerin 1 (MMRN1); Human Multimerin 1 (MMRN1) ELISA Kit; ECM; EMILIN4; GPIa; MMRN; Glycoprotein Ia; Elastin microfibril interface located protein 4; Endothelial cell multimerin; Platelet glycoprotein Ia; 155 kDa platelet multimerin; MMRN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Multimerin 1 (MMRN1).; No significant cross-reactivity or interference between Multimerin 1 (MMRN1) and analogues was observed.
• Sequence Length: 1228

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.65ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Multimerin 1 (MMRN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Multimerin 1 (MMRN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMRN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Multimerin 1 (MMRN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Multimerin 1 (MMRN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Multimerin 1 (MMRN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Multimerin 1 (MMRN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MMRN1 elisa kit

Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 45269141
• NCBI GeneID: 22915
• NCBI Accession #: NP_031377.2
• NCBI GenBank Nucleotide #: NM_007351.2
• UniProt Accession #: Q13201; Q4W5L1; Q6P3T8; Q6ZUL9
• Molecular Weight: 58,170 Da
• NCBI Official Full Name: multimerin-1
• NCBI Official Synonym Full Names: multimerin 1
• NCBI Official Symbol: MMRN1
• NCBI Official Synonym Symbols: ECM; MMRN; GPIa*; EMILIN4
• NCBI Protein Information: multimerin-1
• UniProt Protein Name: Multimerin-1
• Protein Family: Multimerin
• UniProt Gene Name: MMRN1
• UniProt Synonym Gene Names: ECM; EMILIN4; GPIA*; MMRN; Elastin microfibril interfacer 4; p-155; p155
• UniProt Entry Name: MMRN1_HUMAN

NCBI Description

Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin. [provided by RefSeq, Jul 2008]

Uniprot Description

MMRN1: Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein. Deficiency in multimerin-1 due to proteolytic degradation within the platelet alpha granules is associated with an autosomal dominant bleeding disorder (factor V Quebec). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: extracellular region

Biological Process: platelet activation; platelet degranulation; blood coagulation; cell adhesion