Human Mucin-3B (MUC3B) ELISA Kit | MUC3B elisa kit
Human Mucin-3B (MUC3B) ELISA Kit
Gene Names
MUC3B; MUC3; MUC3A
Reactivity
Human
Synonyms
Mucin-3B (MUC3B); Human Mucin-3B (MUC3B) ELISA Kit; MUC3; MUC3A; intestinal mucin MUC3B; mucin 3B; MUC3B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MUC3B. No significant cross-reactivity or interference between Human MUC3B and analogues was observed.
Sequence Length
1237
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MUC3B elisa kit
Description: This assay employs a two-site sandwich ELISA to quantitate MUC3B in samples. An antibody specific for MUC3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The MUC3A gene, originally designated MUC3, encodes a transmembrane mucin-type glycoprotein. A number of consistent single nucleotide changes were observed in different MUC3 cDNAs from a single individual, suggesting the presence of at least 3 different transcripts. this transcript heterogeneity is due to the existence of allelic changes and to tandem duplication of the MUC3 gene.that the second gene, which they designated MUC3B, has the same C-terminal domain and intron-exon structure as that previously described for MUC3. The tandem repeat domain has the same amino acid consensus sequence but shows more substitutions. RT-PCR detected expression of MUC3B in fetal and adult small intestine, fetal and adult colon, and Caco-2 cells.MUC3A and MUC3B, both of which encode membrane-bound mucins with 2 epidermal growth factor-like motifs and a putative transmembrane region.
Overview: The MUC3A gene, originally designated MUC3, encodes a transmembrane mucin-type glycoprotein. A number of consistent single nucleotide changes were observed in different MUC3 cDNAs from a single individual, suggesting the presence of at least 3 different transcripts. this transcript heterogeneity is due to the existence of allelic changes and to tandem duplication of the MUC3 gene.that the second gene, which they designated MUC3B, has the same C-terminal domain and intron-exon structure as that previously described for MUC3. The tandem repeat domain has the same amino acid consensus sequence but shows more substitutions. RT-PCR detected expression of MUC3B in fetal and adult small intestine, fetal and adult colon, and Caco-2 cells.MUC3A and MUC3B, both of which encode membrane-bound mucins with 2 epidermal growth factor-like motifs and a putative transmembrane region.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
131,402 Da
NCBI Official Full Name
Mucin-3B
NCBI Official Synonym Full Names
mucin 3B, cell surface associated
NCBI Official Symbol
MUC3B
NCBI Official Synonym Symbols
MUC3; MUC3A
NCBI Protein Information
mucin-3B
UniProt Protein Name
Mucin-3B
Protein Family
UniProt Gene Name
MUC3B
UniProt Synonym Gene Names
MUC-3BCurated
Uniprot Description
Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces ().
Research Articles on MUC3B
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Product Notes
The Human MUC3B muc3b (Catalog #AAA287529) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287529 ELISA Kit recognizes Human MUC3B. It is sometimes possible for the material contained within the vial of "Mucin-3B (MUC3B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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