Human Monocyte Chemotactic Protein 2 (MCP2) ELISA Kit | MCP2 elisa kit

Human Monocyte Chemotactic Protein 2 (MCP2) ELISA Kit

Cat. Num. AAA2024836

• Gene Names: CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
• Reactivity: Human
• Synonyms: Monocyte Chemotactic Protein 2 (MCP2); Human Monocyte Chemotactic Protein 2 (MCP2) ELISA Kit; CCL8; CCL-8; SCYA8; MCP-2; HC14; Chemokine C-C-Motif Ligand 8; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 8; Monocyte Chemoattractant Protein 2; MCP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 2 (MCP2).; No significant cross-reactivity or interference between Monocyte Chemotactic Protein 2 (MCP2) and analogues was observed.
• Sequence Length: 99

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.128ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 2 (MCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monocyte Chemotactic Protein 2 (MCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MCP2 elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 2916796
• NCBI GeneID: 6355
• UniProt Accession #: P80075; P78388; A0AV77
• Molecular Weight: 11,246 Da
• NCBI Official Full Name: monocyte chemotactic protein-2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 8
• NCBI Official Symbol: CCL8
• NCBI Official Synonym Symbols: HC14; MCP2; MCP-2; SCYA8; SCYA10
• NCBI Protein Information: C-C motif chemokine 8
• UniProt Protein Name: C-C motif chemokine 8
• Protein Family: Meiotic coiled-coil protein
• UniProt Gene Name: CCL8
• UniProt Synonym Gene Names: MCP2; SCYA10; SCYA8; MCP-2
• UniProt Entry Name: CCL8_HUMAN

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; protein kinase activity; phospholipase activator activity

Biological Process: positive regulation of catalytic activity; cellular calcium ion homeostasis; exocytosis; cell-cell signaling; response to virus; calcium ion transport; immune response; signal transduction; inflammatory response; chemotaxis; protein amino acid phosphorylation