Human MMP-10 ELISA Kit | MMP-10 elisa kit

Human MMP-10 (Matrix Metalloproteinase 10) ELISA Kit

Cat. Num. AAA2514092

• Gene Names: MMP21; MMP-21
• Reactivity: Human
• Synonyms: MMP-10; Human MMP-10 (Matrix Metalloproteinase 10) ELISA Kit; MMP-10 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human MMP-10 in samples. No significant cross-reactivity or interference between Human MMP-10 and analogues was observed.
• Sequence Length: 569

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.13-5000pg/mL
• Sensitivity: 46.88pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MMP-10 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MMP-10 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP-10 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MMP-10 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MMP-10. You can calculate the concentration of Human MMP-10 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22218341
• NCBI GeneID: 118856
• NCBI Accession #: NP_671724.1
• NCBI GenBank Nucleotide #: NM_147191.1
• UniProt Accession #: Q8N119; Q5VZP9; Q8NG02
• Molecular Weight: 65,043 Da
• NCBI Official Full Name: matrix metalloproteinase-21 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 21
• NCBI Official Symbol: MMP21
• NCBI Official Synonym Symbols: MMP-21
• NCBI Protein Information: matrix metalloproteinase-21; matrix metalloproteinase 21
• UniProt Protein Name: Matrix metalloproteinase-21
• UniProt Gene Name: MMP21
• UniProt Synonym Gene Names: MMP-21
• UniProt Entry Name: MMP21_HUMAN

NCBI Description

This gene encodes a member of the matrix metalloproteinase family. Proteins in this family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, and disease processes, such as asthma and tumor metastasis. The encoded protein may play an important role in embryogenesis, particularly in neuronal cells, as well as in lymphocyte development and survival. [provided by RefSeq, May 2013]

Uniprot Description

MMP21: May have an important and specific function in tumor progression and embryogenesis. Cleaves alpha-1-antitrypsin. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q26.13

Cellular Component: extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: hemopoietic progenitor cell differentiation; proteolysis