ADM / Adrenomedullin Recombinant Protein | ADM recombinant protein

Recombinant Human ADM / Adrenomedullin Protein (Fc tag)

Cat. Num. AAA2545707

• Gene Names: ADM; AM; PAMP
• Purity: > 93 % as determined by reducing SDS-PAGE.
• Synonyms: ADM / Adrenomedullin; Recombinant Human ADM / Adrenomedullin Protein (Fc tag); AM;PAMP; ADM recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 93 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5%Trehalose, 5% Mannitol, 0.01% Tween 80
• Sequence: Tyr95-Tyr146

• Application Notes: The recombinant human ADM/Fc is a disulfide-linked homodimer. The reduced monomer comprises 340 amino acids and has a predicted molecular mass of 38 kDa. The apparent molecular mass of the protein is approximately 39 in SDS-PAGE under reducing conditions due to glycosylation.
• Species: Human
• Tag: N-Fc
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: It is recommended that sterile water (80uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for ADM recombinant protein

Adrenomedullin consists of 52 amino acids and is a member of the adrenomedullin family. It s a a hypotensive peptide and has 1 intramolecular disulfide bond. It seems that adrenomedullin has a slight homology with the calcitonin generelated peptide. Adrenomedullin has a highly expression in pheochromocytoma and adrenal medulla. It also can be detected in lung, ventricle and kidney tissues. Adrenomedullin and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, adrenomedullin is diuretic and natriuretic, and both adrenomedullin and PAMP inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. It is believed that adrenomedullin functions through combinations of the calcitonin receptor like receptor and receptor activity-modifying proteins complexes, as well as CGRP receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 4501945
• NCBI GeneID: 133
• NCBI Accession #: P35318
• NCBI GenBank Nucleotide #: NM_001124.2
• UniProt Accession #: P35318; Q6FGW2; B2R793; D3DQV3
• Molecular Weight: Mol_Mass: 38kDa; AP_Mol_Mass: 39kDa
• NCBI Official Full Name: ADM
• NCBI Official Synonym Full Names: adrenomedullin
• NCBI Official Symbol: ADM
• NCBI Official Synonym Symbols: AM; PAMP
• NCBI Protein Information: ADM
• UniProt Protein Name: ADM
• Protein Family: ADM
• UniProt Gene Name: ADM
• UniProt Synonym Gene Names: AM; AM; PAMP; ProAM-N20

NCBI Description

The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]

Uniprot Description

ADM: AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Belongs to the adrenomedullin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: protein binding; receptor binding

Biological Process: antibacterial humoral response; antifungal humoral response; blood circulation; cAMP biosynthetic process; cell-cell signaling; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; G-protein coupled receptor internalization; negative regulation of vasoconstriction; positive regulation of cAMP biosynthetic process; progesterone biosynthetic process; receptor internalization; response to yeast; signal transduction; vasculogenesis