Typical Testing Data/Standard Curve (for reference only)
Human Mitotic Arrest Deficient 1 Like Protein 1 (MAD1L1) ELISA Kit | MAD1L1 elisa kit
Human Mitotic Arrest Deficient 1 Like Protein 1 (MAD1L1) ELISA Kit
Gene Names
MAD1L1; MAD1; PIG9; TP53I9; TXBP181
Reactivity
Human
Synonyms
Mitotic Arrest Deficient 1 Like Protein 1 (MAD1L1); Human Mitotic Arrest Deficient 1 Like Protein 1 (MAD1L1) ELISA Kit; HsMAD1; MAD1; PIG9; TP53I9; TXBP181; Mitotic spindle assembly checkpoint protein MAD1; Mitotic checkpoint MAD1 protein homolog; Tax-binding protein 181; MAD1L1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of lysozyme. No significant cross-reactivity or interference between lysozyme and analogues was observed.
Sequence Length
718
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Gallus Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
0.293ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level lysozyme were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level lysozyme were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MAD1L1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of lysozyme in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to lysozyme. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to lysozyme. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain lysozyme, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of lysozyme in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to lysozyme. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to lysozyme. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain lysozyme, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of lysozyme in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,286 Da
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD1 isoform a
NCBI Official Synonym Full Names
mitotic arrest deficient 1 like 1
NCBI Official Symbol
MAD1L1
NCBI Official Synonym Symbols
MAD1; PIG9; TP53I9; TXBP181
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD1
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD1
Protein Family
UniProt Gene Name
MAD1L1
UniProt Synonym Gene Names
MAD1; TXBP181; MAD1-like protein 1; HsMAD1; hMAD1
NCBI Description
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Uniprot Description
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Research Articles on MAD1L1
Similar Products
Product Notes
The Human MAD1L1 mad1l1 (Catalog #AAA2105019) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2105019 ELISA Kit recognizes Human MAD1L1. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the MAD1L1 mad1l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitotic Arrest Deficient 1 Like Protein 1 (MAD1L1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
