Human Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7) ELISA Kit | MAP3K7 elisa kit

Human Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7) ELISA Kit

Cat. Num. AAA2707635

• Gene Names: MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a
• Reactivity: Human
• Synonyms: Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7); Human Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7) ELISA Kit; TAK1; TGF1a; MEKK7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1; MAP3K7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7).; No significant cross-reactivity or interference between Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7) and analogues was observed.
• Sequence Length: 606

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.054ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAP3K7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase Kinase Kinase 7 (MAP3K7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21735562
• NCBI GeneID: 6885
• NCBI GenBank Nucleotide #: NP_663304.1
• UniProt Accession #: O43318
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7 isoform B
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Symbol: MAP3K7
• NCBI Official Synonym Symbols: CSCF; FMD2; TAK1; MEKK7; TGF1a
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K7
• UniProt Synonym Gene Names: TAK1; TGF-beta-activated kinase 1
• UniProt Entry Name: M3K7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TAK1: a protein kinase of the MLK family. Mediates signal transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. Also activates MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced isoforms have been reported. Three splice variant isoforms have been described.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; TAK1 subfamily

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; endosome membrane; IkappaB kinase complex; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; positive regulation of T cell cytokine production; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; transforming growth factor beta receptor signaling pathway; JNK cascade; angiogenesis; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-2 production; toll-like receptor signaling pathway; innate immune response; positive regulation of T cell activation; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; neural tube formation