Human Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) ELISA Kit | MAP1LC3a elisa kit

Human Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) ELISA Kit

Cat. Num. AAA451127

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Reactivity: Human
• Synonyms: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a); Human Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) ELISA Kit; MAP1ALC3; MAP1BLC3; LC3; LC3A; ATG8E; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1LC3a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAP1LC3a. No significant cross-reactivity or interference between MAP1LC3a and analogues was observed.
• Sequence Length: 121

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.063ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MAP1LC3a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MAP1LC3a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAP1LC3a elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MAP1LC3a in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP1LC3a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MAP1LC3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAP1LC3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MAP1LC3a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14210522
• NCBI GeneID: 84557
• NCBI Accession #: NP_115903.1
• NCBI GenBank Nucleotide #: NM_032514.3
• UniProt Accession #: Q9H492; Q9BXW5; E1P5P4; E1P5P5
• Molecular Weight: 14,493 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3A isoform a
• NCBI Official Synonym Full Names: microtubule associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).