Human Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) ELISA Kit | MTHFD1 elisa kit

Human Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) ELISA Kit

Cat. Num. AAA9311869

• Gene Names: MTHFD1; MTHFC; MTHFD
• Reactivity: Human
• Synonyms: Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1); Human Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) ELISA Kit; MTHFD1 elisa kit

• Reactivity: Human

• Samples: Undiluted original Human serum, plasma or tissue homogenates samples
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625ng/ml-20ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MTHFD1 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used for determine the level of MTHFD1 (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 38014238
• NCBI GeneID: 4522
• UniProt Accession #: P11586; Q86VC9; Q9BVP5; B2R5Y2
• Molecular Weight: 101,559 Da
• NCBI Official Full Name: MTHFD1 protein, partial
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• NCBI Official Symbol: MTHFD1
• NCBI Official Synonym Symbols: MTHFC; MTHFD
• NCBI Protein Information: C-1-tetrahydrofolate synthase, cytoplasmic; C1-THF synthase; cytoplasmic C-1-tetrahydrofolate synthase; 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase
• UniProt Protein Name: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Gene Name: MTHFD1
• UniProt Synonym Gene Names: MTHFC; MTHFD; C1-THF synthase
• UniProt Entry Name: C1TC_HUMAN

NCBI Description

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]

Uniprot Description

MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.

Protein type: EC 1.5.1.5; Oxidoreductase; Hydrolase; EC 6.3.4.3; Cofactor and Vitamin Metabolism - one carbon pool by folate; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.5.4.9; Ligase

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: mitochondrion; membrane; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; methylenetetrahydrofolate dehydrogenase activity

Biological Process: histidine biosynthetic process; vitamin metabolic process; folic acid and derivative biosynthetic process; methionine biosynthetic process; purine nucleotide biosynthetic process; one-carbon compound metabolic process; folic acid metabolic process; water-soluble vitamin metabolic process

Disease: Neural Tube Defects, Folate-sensitive