Human Metallothionein-3 (MT3) ELISA Kit | MT3 elisa kit

Human Metallothionein-3 (MT3) ELISA Kit

Cat. Num. AAA9715170

• Gene Names: MT3; GIF; GIFB; GRIF; ZnMT3
• Reactivity: Human
• Synonyms: Metallothionein-3 (MT3); Human Metallothionein-3 (MT3) ELISA Kit; MT3; GIF; GIFB; GRIF; metallothionein 3 (growth inhibitory factor (neurotrophic)); MT3 elisa kit

• Reactivity: Human
• Specificity: Human Metallothionein-3 (MT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT3. No significant cross-reactivity or interference between Human MT3 and analogues was observed.
• Sequence Length: 68

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Metallothionein-3 (MT3) Standard Curve.)

Related Product Information for MT3 elisa kit

Description: This Human Metallothionein-3 (MT3) ELISA Kit employs a two-site sandwich ELISA to quantitate MT3 in samples. An antibody specific for MT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MT3 (Metallothionein 3) is a Protein Coding gene, and is affiliated with the lncRNA class. Diseases associated with MT3 include Alzheimer Disease and Amyotrophic Lateral Sclerosis 1. Among its related pathways are Metabolism and Response to metal ions. GO annotations related to this gene include drug binding and antioxidant activity. Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.

NCBI and Uniprot Product Information

• NCBI GI #: 5174762
• NCBI GeneID: 4504
• NCBI Accession #: NP_005945.1
• NCBI GenBank Nucleotide #: NP_005945.1
• UniProt Accession #: P25713
• NCBI Official Full Name: metallothionein-3
• NCBI Official Synonym Full Names: metallothionein 3
• NCBI Official Symbol: MT3
• NCBI Official Synonym Symbols: GIF; GIFB; GRIF; ZnMT3
• NCBI Protein Information: metallothionein-3
• UniProt Protein Name: Metallothionein-3
• Protein Family: Metallothionein
• UniProt Gene Name: MT3
• UniProt Synonym Gene Names: MT-3; GIF; MT-III
• UniProt Entry Name: MT3_HUMAN

NCBI Description

This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. This gene family member displays tissue-specific expression, and contains a threonine insert near its N-terminus and a glutamate-rich hexapeptide insert near its C-terminus relative to the proteins encoded by other gene family members. It plays an important role in zinc and copper homeostasis, and is induced under hypoxic conditions. The encoded protein is a growth inhibitory factor, and reduced levels of the protein are observed in the brains of individuals with some metal-linked neurodegenerative disorders such as Alzheimer's disease. [provided by RefSeq, Sep 2017]

Uniprot Description

MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family.

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; intracellular; inclusion body

Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; caspase inhibitor activity; drug binding; protein kinase activator activity; cadmium ion binding

Biological Process: positive regulation of catalytic activity; negative regulation of autophagy; removal of superoxide radicals; positive regulation of transcription, DNA-dependent; negative regulation of axon extension; negative regulation of caspase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; zinc ion homeostasis; cadmium ion homeostasis; cellular lipid catabolic process; histone modification; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cholesterol catabolic process; regulation of response to food; protein stabilization; activation of protein kinase B; cellular zinc ion homeostasis; zinc ion transport; regulation of protein amino acid glycosylation; leptin-mediated signaling pathway; astrocyte development; cell proliferation; cellular metal ion homeostasis; protein import into nucleus, translocation; negative regulation of oxidoreductase activity; energy reserve metabolic process; response to hypoxia; negative regulation of cell growth; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis