Human Melanoma Associated ME20 (ME20M) ELISA Kit | ME20M elisa kit

Human Melanoma Associated ME20 (ME20M) ELISA Kit

Cat. Num. AAA455192

• Gene Names: PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• Reactivity: Human
• Synonyms: Melanoma Associated ME20 (ME20M); Human Melanoma Associated ME20 (ME20M) ELISA Kit; PMEL; GP100; SI; SIL; SILV; Pmel17; Silver Homolog; Premelanosome Protein; Silver Locus Protein Homolog; 95 kDa melanocyte-specific secreted glycoprotein; ME20M elisa kit

• Reactivity: Human
• Sequence Length: 575

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• Intra Assay: CV<10%
• Inter Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 318037593
• NCBI GeneID: 6490
• NCBI Accession #: NP_001186982.1
• NCBI GenBank Nucleotide #: NM_001200053.1
• UniProt Accession #: P40967; Q12763; Q14448; Q14817; Q16565; B3KS57; B7Z6D7
• Molecular Weight: 66,729 Da
• NCBI Official Full Name: melanocyte protein PMEL isoform 2
• NCBI Official Synonym Full Names: premelanosome protein
• NCBI Official Symbol: PMEL
• NCBI Official Synonym Symbols: P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• NCBI Protein Information: melanocyte protein PMEL
• UniProt Protein Name: Melanocyte protein PMEL
• UniProt Gene Name: PMEL
• UniProt Synonym Gene Names: D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S

NCBI Description

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Uniprot Description

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.