Typical Standard Curve/Testing Data
Human Melanocyte stimulating hormone receptor (MC1R) ELISA Kit | MC1R elisa kit
Human Melanocyte stimulating hormone receptor (MC1R) ELISA Kit
Reactivity
Human
Synonyms
Melanocyte stimulating hormone receptor (MC1R); Human Melanocyte stimulating hormone receptor (MC1R) ELISA Kit; MC1R elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for MC1R elisa kit
Intended Uses: This MC1R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MC1R. This ELISA kit for research use only!
Principle of the Assay: MC1R ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MC1R antibody and an MC1R-HRP conjugate. The assay sample and buffer are incubated together with MC1R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MC1R concentration since MC1R from samples and MC1R-HRP conjugate compete for the anti-MC1R antibody binding site. Since the number of sites is limited, as more sites are occupied by MC1R from the sample, fewer sites are left to bind MC1R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MC1R concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MC1R ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MC1R antibody and an MC1R-HRP conjugate. The assay sample and buffer are incubated together with MC1R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MC1R concentration since MC1R from samples and MC1R-HRP conjugate compete for the anti-MC1R antibody binding site. Since the number of sites is limited, as more sites are occupied by MC1R from the sample, fewer sites are left to bind MC1R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MC1R concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
34,895 Da
NCBI Official Full Name
melanocyte stimulating hormone receptor
UniProt Protein Name
Melanocyte-stimulating hormone receptor
Protein Family
UniProt Gene Name
MC1R
UniProt Synonym Gene Names
MSHR; MSH-R; MC1-R
UniProt Entry Name
MSHR_RANTA
Uniprot Description
Receptor for MSH (alpha, beta) and ACTH. Does not seem to be active with gamma-MSH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Could be involved in spermatogenesis.
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Product Notes
The Human MC1R mc1r (Catalog #AAA7252387) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7252387 ELISA Kit recognizes Human MC1R. It is sometimes possible for the material contained within the vial of "Melanocyte stimulating hormone receptor (MC1R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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