Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM) ELISA Kit | ACADM elisa kit

Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM) ELISA Kit

Cat. Num. AAA2600366

• Gene Names: ACADM; MCAD; ACAD1; MCADH
• Reactivity: Human
• Synonyms: Medium-chain specific acyl-CoA dehydrogenase; mitochondrial (ACADM); Human Medium-chain specific acyl-CoA dehydrogenase; mitochondrial (ACADM) ELISA Kit; ACADM elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 425

• Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant ACADM concentration.
• Assay Type: Sandwich
• Detection Range: 200 ng/ml-3.12 ng/ml
• Sensitivity: 0.6 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for ACADM elisa kit

Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ACADM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 187960098
• NCBI GeneID: 34
• NCBI Accession #: NP_001120800.1
• NCBI GenBank Nucleotide #: NM_001127328.2
• Molecular Weight: 47,020 Da
• NCBI Official Full Name: medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform b
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase, C-4 to C-12 straight chain
• NCBI Official Symbol: ACADM
• NCBI Official Synonym Symbols: MCAD; ACAD1; MCADH
• NCBI Protein Information: medium-chain specific acyl-CoA dehydrogenase, mitochondrial; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• UniProt Protein Name: Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
• Protein Family: Medium-chain specific acyl-CoA dehydrogenase
• UniProt Gene Name: ACADM
• UniProt Synonym Gene Names: MCAD
• UniProt Entry Name: ACADM_HUMAN

NCBI Description

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACADM: This enzyme is specific for acyl chain lengths of 4 to 16. Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD). It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. Belongs to the acyl-CoA dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.3.8.7; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - propanoate; Oxidoreductase; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: mitochondrion; mitochondrial matrix; axon; nucleus

Molecular Function: identical protein binding; acyl-CoA dehydrogenase activity; FAD binding

Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation; medium-chain fatty acid catabolic process; cellular lipid metabolic process; medium-chain fatty acid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; carnitine biosynthetic process

Disease: Acyl-coa Dehydrogenase, Medium-chain, Deficiency Of