Human MBL ELISA Kit | MBL elisa kit

MBL, Human, ELISA Kit

Cat. Num. AAA584558

• Reactivity: Human
• Synonyms: MBL; Human; ELISA Kit; Mannose- or mannan-binding protein (MBP); MBL elisa kit

• Reactivity: Human
• Sequence Length: 333

• Application Notes: Application Notes: The human MBL (Lectin assay) ELISA has been developed for the quantitative measurement of natural functional, mannan binding, human MBL in serum, plasma and culture medium.
• Detection Level: 0.41 ng/ml
• Standard Range: 0.41-100 ng/ml
• Working Volume: 100 ul/well
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for MBL elisa kit

Product Description: Mannose Binding Lectin (MBL) is a C-type lectin that is an important element in innate immunity. MBL belongs to the collectin family of proteins that consists of a collagen-like domain and a carbohydrate recognition domain (CRD). Through this, MBL recognizes carbohydrates (e.g. mannose and N-acetylglucosamine) on pathogens. MBL forms several sizes of oligomers that are composed of subunits of three identical 32 kDa polypeptides. MBL is a pattern recognition receptor, and upon recognition of the infectious agent, MBL triggers the activation of the lectin-complement pathway through attached C1r/C1s-like serine proteases, designated MASP. The MBL-MASP complex proteolytically cleaves C4, C2 and C3. The lectin pathway is antibody and C1q-independent and is, therefore, independent of the classical and alternative complement activation pathway. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Normal human plasma contains MBL concentrations ranging from 10 to 5,000 ng/ml. Up to 12% of healthy Caucasian blood donors have MBL concentrations below 100 ng/ml. Low plasma concentrations have been associated with an inherited defect in opsonization. The MBL concentration is enhanced in infectious diseases. Measuring of MBL is indicated in recurrent infections (especially in children), primary/secondary immunodeficiencies, artherosclerosis/coronary heart disease, cystic fibrosis, transplantation, autoimmune diseases (SLE/Rheumatoid arthritis) and habitual abortion. MBL measurement is not affected by the presence of antibodies against mannan. In the human MBL assay, any influence of the classical pathway has been eliminated by using a special MBL-binding buffer, which inhibits the binding of C1q to immunocomplexes and disrupts the C1 complex, while leaving the function of the MBL complex intact.
Principle of the assay: The human MBL ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4 hours.

References

1. Kirkpatrick, B et al; Serum mannose-binding lectin deficiency is associated with cryptosporidiosis in young Haitian children. Clin Infect Dis 2006, 43: 289
2. Druszczynska, M et al; Tuberculosis bacilli still posing a threat. Polymorphism of genes regulating anti-mycobacterial properties of macrophages. Pol J Microbiol 2006, 55: 7
3. Wang, X et al; Mannose-binding lectin gene polymorphisms and the development of coal workers pneumoconiosis in Japan. Am J Ind Med 2008, 51: 548
4. Ribeiro, L et al; Serum mannose-binding lectin levels are linked with respiratory syncytial virus (RSV) disease. J clin immunol 2008, 28: 166
5. Koutsounaki, E et al; Mannose-binding lectin MBL2 gene polymorphisms and the outcome of hepatitis C virus-infected patients. J Clin Immunol 2008, 28: 495

NCBI and Uniprot Product Information

• NCBI GI #: 154353611
• Molecular Weight: 35,875 Da
• NCBI Official Full Name: Mbl
• UniProt Protein Name: Mbl
• Protein Family: MreB-like protein
• UniProt Gene Name: mbl
• UniProt Synonym Gene Names: RBAM_033610
• UniProt Entry Name: A7Z9L4_BACA2