Human Matrix Metalloproteinase-21 (MMP21) ELISA Kit | MMP21 elisa kit

Human Matrix Metalloproteinase-21 (MMP21) ELISA Kit

Cat. Num. AAA9715257

• Gene Names: MMP21; HTX7; MMP-21
• Reactivity: Human
• Synonyms: Matrix Metalloproteinase-21 (MMP21); Human Matrix Metalloproteinase-21 (MMP21) ELISA Kit; MMP21; matrix metalloproteinase 21; MMP21 elisa kit

• Reactivity: Human
• Specificity: Human Matrix metalloproteinase-21 (MMP21) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP21. No significant cross-reactivity or interference between Human MMP21 and analogues was observed.
• Sequence Length: 569

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Matrix metalloproteinase-21 (MMP21) Standard Curve.)

Related Product Information for MMP21 elisa kit

Description: This Human Matrix metalloproteinase-21 (MMP21) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP21 in samples. An antibody specific for MMP21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP21 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MMP21 encodes a member of the matrix metalloproteinase family. Proteins in this family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, and disease processes, such as asthma and tumor metastasis. The encoded protein may play an important role in embryogenesis, particularly in neuronal cells, as well as in lymphocyte development and survival. 

NCBI and Uniprot Product Information

• NCBI GI #: 22218341
• NCBI GeneID: 118856
• NCBI Accession #: NP_671724.1
• NCBI GenBank Nucleotide #: NP_671724.1
• UniProt Accession #: Q8N119
• NCBI Official Full Name: matrix metalloproteinase-21 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 21
• NCBI Official Symbol: MMP21
• NCBI Official Synonym Symbols: HTX7; MMP-21
• NCBI Protein Information: matrix metalloproteinase-21
• UniProt Protein Name: Matrix metalloproteinase-21
• Protein Family: Matrix metallopeptidase
• UniProt Gene Name: MMP21
• UniProt Synonym Gene Names: MMP-21
• UniProt Entry Name: MMP21_HUMAN

NCBI Description

This gene encodes a member of the matrix metalloproteinase family. Proteins in this family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, and disease processes, such as asthma and tumor metastasis. The encoded protein may play an important role in embryogenesis, particularly in neuronal cells, as well as in lymphocyte development and survival. [provided by RefSeq, May 2013]