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Typical Testing Data/Standard Curve (for reference only)

Human MARCKS Related Protein (MARCKSL1) ELISA Kit | MARCKSL1 elisa kit

Human MARCKS Related Protein (MARCKSL1) ELISA Kit

Gene Names
MARCKSL1; F52; MLP; MRP; MLP1; MACMARCKS
Reactivity
Human
Synonyms
MARCKS Related Protein (MARCKSL1); Human MARCKS Related Protein (MARCKSL1) ELISA Kit; MLP1; MRP; MLP; F52; MACMARCKS; MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate; MARCKSL1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MARCKS Related Protein (MARCKSL1).
No significant cross-reactivity or interference between MARCKS Related Protein (MARCKSL1) and analogues was observed.
Sequence Length
195
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.117ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level MARCKS Related Protein (MARCKSL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level MARCKS Related Protein (MARCKSL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MARCKSL1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MARCKS Related Protein (MARCKSL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MARCKS Related Protein (MARCKSL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MARCKS Related Protein (MARCKSL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MARCKS Related Protein (MARCKSL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MARCKSL1 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,529 Da
NCBI Official Full Name
MARCKS-related protein
NCBI Official Synonym Full Names
MARCKS-like 1
NCBI Official Symbol
MARCKSL1
NCBI Official Synonym Symbols
F52; MLP; MRP; MLP1; MACMARCKS
NCBI Protein Information
MARCKS-related protein
UniProt Protein Name
MARCKS-related protein
Protein Family
UniProt Gene Name
MARCKSL1
UniProt Synonym Gene Names
MLP; MRP; Mac-MARCKS; MacMARCKS
UniProt Entry Name
MRP_HUMAN

NCBI Description

This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10. [provided by RefSeq, Jun 2012]

Uniprot Description

MLP: Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems. Belongs to the MARCKS family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calmodulin binding; actin binding

Biological Process: positive regulation of cell proliferation

Research Articles on MARCKSL1

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Product Notes

The Human MARCKSL1 marcksl1 (Catalog #AAA2020791) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020791 ELISA Kit recognizes Human MARCKSL1. It is sometimes possible for the material contained within the vial of "MARCKS Related Protein (MARCKSL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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