Human Mannose Binding Lectin (MBL) ELISA Kit | MBL elisa kit

Human Mannose Binding Lectin (MBL) ELISA Kit

Cat. Num. AAA2022260

• Gene Names: MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• Reactivity: Human
• Synonyms: Mannose Binding Lectin (MBL); Human Mannose Binding Lectin (MBL) ELISA Kit; MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2; Soluble(Opsonic Defect); MBL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL).; No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.
• Sequence Length: 38

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.069ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MBL elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Binding Lectin (MBL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Binding Lectin (MBL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Binding Lectin (MBL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Binding Lectin (MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MBL elisa kit

Signal transduction; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 169335948
• NCBI GeneID: 4153
• Molecular Weight: 3,557 Da
• NCBI Official Full Name: mannose binding lectin, partial
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2, soluble
• NCBI Official Symbol: MBL2
• NCBI Official Synonym Symbols: MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• NCBI Protein Information: mannose-binding protein C
• UniProt Protein Name: Mannose binding lectin
• Protein Family: MreB-like protein
• UniProt Gene Name: MBL2
• UniProt Entry Name: B1PN75_HUMAN

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; cell surface; collagen; extracellular region

Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; opsonization; response to oxidative stress; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation

Disease: Mannose-binding Protein Deficiency