Human Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) ELISA Kit | ME2 elisa kit

Human Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) ELISA Kit

Cat. Num. AAA2022617

• Gene Names: ME2; ODS1
• Reactivity: Human
• Synonyms: Malic Enzyme 2; NADP+ Dependent; Mitochondrial (ME2); Human Malic Enzyme 2; Mitochondrial (ME2) ELISA Kit; ME2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2).; No significant cross-reactivity or interference between Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) and analogues was observed.
• Sequence Length: 479

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ME2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ME2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 119583385
• NCBI GeneID: 4200
• UniProt Accession #: P23368; Q9BWL6; Q9BYG1; Q9H4B2; B2R8J2
• Molecular Weight: 53,586 Da
• NCBI Official Full Name: malic enzyme 2, NAD(+)-dependent, mitochondrial, isoform CRA_b
• NCBI Official Synonym Full Names: malic enzyme 2, NAD(+)-dependent, mitochondrial
• NCBI Official Symbol: ME2
• NCBI Official Synonym Symbols: ODS1
• NCBI Protein Information: NAD-dependent malic enzyme, mitochondrial
• UniProt Protein Name: NAD-dependent malic enzyme, mitochondrial
• UniProt Gene Name: ME2
• UniProt Synonym Gene Names: NAD-ME
• UniProt Entry Name: MAOM_HUMAN

NCBI Description

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

ME2: a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Protein type: Oxidoreductase; Carbohydrate Metabolism - pyruvate; EC 1.1.1.38; Mitochondrial

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix

Molecular Function: malate dehydrogenase (decarboxylating) activity; electron carrier activity; metal ion binding; NAD binding; oxaloacetate decarboxylase activity

Biological Process: malate metabolic process

Disease: Opioid Dependence, Susceptibility To, 1; Epilepsy, Idiopathic Generalized