Human M-CSFR ELISA Kit | M-CSFR elisa kit

M-CSFR (Human) OmniKine ELISA Kit

Cat. Num. AAA9501978

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• Reactivity: Human
• Synonyms: M-CSFR; M-CSFR (Human) OmniKine ELISA Kit; Macrophage colony-stimulating factor 1 receptor; Proto-oncogene c-Fms; CD115; M-CSFR elisa kit

• Reactivity: Human
• Specificity: The OmniKine Human M-CSFR ELISA is capable of recognizing both recombinant and naturally produced Human M-CSFR proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.; Human: GM-CSF, PDGF-R, PDGF-R/Fc; Murine: M-CSF
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 972

• Samples: Plasma, serum, lysate
• Assay Type: Sandwich ELISA
• Detection Range: 63-4000 pg/ml
• Sensitivity: 63-4000 pg/ml
• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human M-CSFR ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human M-CSFR proteins within the range of 63-4000 pg/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 569026720
• NCBI GeneID: 1436
• NCBI Accession #: NP_001275634.1
• NCBI GenBank Nucleotide #: NM_001288705.2
• UniProt Accession #: P07333; Q6LDW5; Q6LDY4; Q86VW7; B5A955; D3DQG2
• Molecular Weight: 33,248 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 receptor
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R

NCBI Description

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma. [provided by RefSeq, Mar 2017]

Uniprot Description

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell surface; CSF1-CSF1R complex; integral component of plasma membrane; intracellular membrane-bound organelle; nucleoplasm; plasma membrane

Molecular Function: ATP binding; cytokine binding; macrophage colony-stimulating factor receptor activity; protein binding; protein homodimerization activity; protein phosphatase binding

Biological Process: axon guidance; cell proliferation; cellular response to cytokine stimulus; cytokine-mediated signaling pathway; forebrain neuron differentiation; hemopoiesis; inflammatory response; innate immune response; intercellular junction maintenance; macrophage colony-stimulating factor signaling pathway; macrophage differentiation; monocyte differentiation; multicellular organism development; negative regulation of apoptosis; negative regulation of cell proliferation; olfactory bulb development; osteoclast differentiation; peptidyl-tyrosine phosphorylation; phosphatidylinositol metabolic process; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of chemokine secretion; positive regulation of ERK1 and ERK2 cascade; positive regulation of protein phosphorylation; positive regulation of protein serine/threonine kinase activity; positive regulation of tyrosine phosphorylation of STAT protein; protein autophosphorylation; regulation of actin cytoskeleton reorganization; regulation of bone resorption; regulation of cell shape; ruffle organization; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Leukoencephalopathy, Diffuse Hereditary, With Spheroids