Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lysyl oxidase homolog 4 (LOXL4) Standard Curve.)
Human Lysyl Oxidase Homolog 4 (LOXL4) ELISA Kit | LOXL4 elisa kit
Human Lysyl Oxidase Homolog 4 (LOXL4) ELISA Kit
Gene Names
LOXL4; LOXC
Reactivity
Human
Synonyms
Lysyl Oxidase Homolog 4 (LOXL4); Human Lysyl Oxidase Homolog 4 (LOXL4) ELISA Kit; LOXL4; FLJ21889; LOXC; lysyl oxidase homolog 4; lysyl oxidase related C; LOXL4 elisa kit
Reactivity
Human
Specificity
Human Lysyl oxidase homolog 4 (LOXL4) ELISA Kit has high sensitivity and excellent specificity for detection of Human LOXL4. No significant cross-reactivity or interference between Human LOXL4 and analogues was observed.
Sequence Length
756
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lysyl oxidase homolog 4 (LOXL4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lysyl oxidase homolog 4 (LOXL4) Standard Curve.)
Related Product Information for LOXL4 elisa kit
Description: This Human Lysyl oxidase homolog 4 (LOXL4) ELISA Kit employs a two-site sandwich ELISA to quantitate LOXL4 in samples. An antibody specific for LOXL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LOXL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOXL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOXL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lysyl oxidase homolog 4 is a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.
Background: Lysyl oxidase homolog 4 is a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lysyl oxidase homolog 4
NCBI Official Synonym Full Names
lysyl oxidase like 4
NCBI Official Symbol
LOXL4
NCBI Official Synonym Symbols
LOXC
NCBI Protein Information
lysyl oxidase homolog 4
UniProt Protein Name
Lysyl oxidase homolog 4
Protein Family
UniProt Gene Name
LOXL4
UniProt Synonym Gene Names
LOXC
NCBI Description
This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]
Uniprot Description
May modulate the formation of a collagenous extracellular matrix.
Research Articles on LOXL4
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Product Notes
The Human LOXL4 loxl4 (Catalog #AAA9715461) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715461 ELISA Kit recognizes Human LOXL4. It is sometimes possible for the material contained within the vial of "Lysyl Oxidase Homolog 4 (LOXL4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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