Human Lysine-specific demethylase 5D (KDM5D) ELISA Kit | KDM5D elisa kit
Human Lysine-specific demethylase 5D (KDM5D) ELISA Kit
Gene Names
KDM5D; SMCY; JARID1D
Reactivity
Human
Synonyms
Lysine-specific demethylase 5D (KDM5D); Human Lysine-specific demethylase 5D (KDM5D) ELISA Kit; HY; HYA; JARID1D; KIAA0234; SMCY; Jumonji; AT rich interactive domain 1D (RBP2-like); SMC homolog; Y chromosome; Smcy homolog; Y-linked; histocompatibility Y antigen; jumonji; KDM5D elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human KDM5D. No significant cross-reactivity or interference between Human KDM5D and analogues was observed.
Sequence Length
1545
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for KDM5D elisa kit
Description: This assay employs a two-site sandwich ELISA to quantitate KDM5D in samples. An antibody specific for KDM5D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KDM5D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDM5D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDM5D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: KDM5D encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants.It was clustered with the Ube1y and the Zfy1 genes in a segment of approximately 250 kb. A homologous gene, Smcx (JARID1C), was found on the X chromosome. Expression of Smcy was detected in all male tissues and expression of Smcx in all male and female tissues tested. Remarkably, the expression of both genes was detected in pools of mouse preimplantation embryos as early as the 2-cell stage. They found that Smcy, like Sry and Ube1y, has been conserved on the Y chromosome since the divergence of metatherian and eutherian mammals some 120 million years ago.
Overview: KDM5D encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants.It was clustered with the Ube1y and the Zfy1 genes in a segment of approximately 250 kb. A homologous gene, Smcx (JARID1C), was found on the X chromosome. Expression of Smcy was detected in all male tissues and expression of Smcx in all male and female tissues tested. Remarkably, the expression of both genes was detected in pools of mouse preimplantation embryos as early as the 2-cell stage. They found that Smcy, like Sry and Ube1y, has been conserved on the Y chromosome since the divergence of metatherian and eutherian mammals some 120 million years ago.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
175,621 Da
NCBI Official Full Name
lysine-specific demethylase 5D
NCBI Official Symbol
KDM5D
NCBI Official Synonym Symbols
SMCY; JARID1D
NCBI Protein Information
lysine-specific demethylase 5D
UniProt Protein Name
Lysine-specific demethylase 5D
Protein Family
UniProt Gene Name
KDM5D
UniProt Synonym Gene Names
JARID1D; SMCY
Uniprot Description
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May play a role in spermatogenesis. Involved in transcriptional repression of diverse metastasis-associated genes; in this function seems to cooperate with ZMYND8. Suppresses prostate cancer cell invasion. Regulates androgen receptor (AR) transcriptional activity by demethylating H3K4me3 active transcription marks ().
Similar Products
Product Notes
The Human KDM5D kdm5d (Catalog #AAA287571) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287571 ELISA Kit recognizes Human KDM5D. It is sometimes possible for the material contained within the vial of "Lysine-specific demethylase 5D (KDM5D), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
