Human Lymphotactin (Lptn) ELISA Kit | Lptn elisa kit

Human Lymphotactin (Lptn) ELISA Kit

Cat. Num. AAA2702484

• Gene Names: XCL1; LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
• Reactivity: Human
• Synonyms: Lymphotactin (Lptn); Human Lymphotactin (Lptn) ELISA Kit; XCL1; SCYC1; LTN; ATAC; SCM1-A; SCM1a; LPTN; Lymphotaxin; Chemokine C-Motif Ligand 1; Small Inducible Cytokine Subfamily C; Member 1; XC chemokine ligand 1; Lptn elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lymphotactin (Lptn).; No significant cross-reactivity or interference between Lymphotactin (Lptn) and analogues was observed.
• Sequence Length: 114

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 5.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphotactin (Lptn) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphotactin (Lptn) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Lptn elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphotactin (Lptn). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphotactin (Lptn). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphotactin (Lptn), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphotactin (Lptn) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506853
• NCBI GeneID: 6375
• NCBI Accession #: NP_002986.1
• NCBI GenBank Nucleotide #: NM_002995.2
• UniProt Accession #: P47992; Q52MA8
• Molecular Weight: 12,517 Da
• NCBI Official Full Name: lymphotactin
• NCBI Official Synonym Full Names: X-C motif chemokine ligand 1
• NCBI Official Symbol: XCL1
• NCBI Official Synonym Symbols: LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
• NCBI Protein Information: lymphotactin
• UniProt Protein Name: Lymphotactin
• UniProt Gene Name: XCL1
• UniProt Synonym Gene Names: LTN; SCYC1

NCBI Description

This antimicrobial gene encodes a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation. The encoded protein is a member of the C-chemokine subfamily, retaining only two of four cysteines conserved in other chemokines, and is thought to be specifically chemotactic for T cells. This gene and a closely related family member are located on the long arm of chromosome 1. [provided by RefSeq, Sep 2014]

Uniprot Description

XCL1: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: cell; extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; protein homodimerization activity

Biological Process: cell-cell signaling; G-protein coupled receptor signaling pathway; monocyte chemotaxis; negative regulation of interferon-gamma production; negative regulation of interleukin-2 production; negative regulation of T cell cytokine production; negative regulation of T-helper 1 type immune response; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of interleukin-10 production; positive regulation of leukocyte chemotaxis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell cytokine production; positive regulation of T cell mediated cytotoxicity; regulation of inflammatory response; release of sequestered calcium ion into cytosol; response to virus; signal transduction