Human Lumican (LUM) ELISA Kit | LUM elisa kit

Human Lumican (LUM) ELISA Kit

Cat. Num. AAA2019607

• Gene Names: LUM; LDC; SLRR2D
• Reactivity: Human
• Synonyms: Lumican (LUM); Human Lumican (LUM) ELISA Kit; LDC; SLRR2D; KSPG lumican; Keratan sulfate proteoglycan lumican; LUM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lumican (LUM).; No significant cross-reactivity or interference between Lumican (LUM) and analogues was observed.
• Sequence Length: 338

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lumican (LUM) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lumican (LUM) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LUM elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lumican (LUM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lumican (LUM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lumican (LUM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lumican (LUM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LUM elisa kit

Metabolic pathway; Tumor immunity; Infection immunity; Bone metabolism; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 4505047
• NCBI GeneID: 4060
• NCBI Accession #: NP_002336.1
• NCBI GenBank Nucleotide #: NM_002345.3
• UniProt Accession #: P51884; Q96QM7; B2R6R5
• Molecular Weight: 38,429 Da
• NCBI Official Full Name: lumican
• NCBI Official Synonym Full Names: lumican
• NCBI Official Symbol: LUM
• NCBI Official Synonym Symbols: LDC; SLRR2D
• NCBI Protein Information: lumican
• UniProt Protein Name: Lumican
• Protein Family: Lumican
• UniProt Gene Name: LUM
• UniProt Synonym Gene Names: LDC; SLRR2D; KSPG lumican
• UniProt Entry Name: LUM_HUMAN

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Uniprot Description

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; lysosomal lumen; fibrillar collagen; Golgi lumen; extracellular region

Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent

Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; visual perception; glycosaminoglycan metabolic process; cartilage development; keratan sulfate biosynthetic process; carbohydrate metabolic process; pathogenesis; positive regulation of transcription from RNA polymerase II promoter; keratan sulfate catabolic process; response to organic cyclic substance