Human LUM ELISA Kit | LUM elisa kit

Human LUM (Lumican) ELISA Kit

Cat. Num. AAA2511791

• Gene Names: LUM; LDC; SLRR2D
• Reactivity: Human
• Synonyms: LUM; Human LUM (Lumican) ELISA Kit; LUM elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human LUM. No significant cross-reactivity or interference between Human LUM and analogues was observed.
• Sequence Length: 338

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LUM concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LUM elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LUM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LUM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LUM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LUM. You can calculate the concentration of LUM in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505047
• NCBI GeneID: 4060
• NCBI Accession #: NP_002336.1
• NCBI GenBank Nucleotide #: NM_002345.3
• UniProt Accession #: P51884; Q96QM7; B2R6R5
• Molecular Weight: 38,429 Da
• NCBI Official Full Name: lumican
• NCBI Official Synonym Full Names: lumican
• NCBI Official Symbol: LUM
• NCBI Official Synonym Symbols: LDC; SLRR2D
• NCBI Protein Information: lumican; KSPG lumican; keratan sulfate proteoglycan lumican; lumican proteoglycan
• UniProt Protein Name: Lumican
• Protein Family: Lumican
• UniProt Gene Name: LUM
• UniProt Synonym Gene Names: LDC; SLRR2D; KSPG lumican
• UniProt Entry Name: LUM_HUMAN

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Uniprot Description

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; proteinaceous extracellular matrix; fibrillar collagen; Golgi lumen; extracellular region

Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent

Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; glycosaminoglycan metabolic process; visual perception; cartilage development; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; positive regulation of transcription from RNA polymerase II promoter; keratan sulfate catabolic process; response to organic cyclic substance