Human LOX-1 ELISA Kit | LOX-1 elisa kit

Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit

Cat. Num. AAA2504732

• Gene Names: Olr1; LOX-1; Oldr1; Oldlr1
• Reactivity: Human
• Synonyms: LOX-1; Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit; LOX-1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human LOX-1 in samples. No significant cross-reactivity or interference between Human LOX-1 and analogues was observed.
• Sequence Length: 364

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.16-10ng/mL
• Sensitivity: 0.1ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human LOX-1 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human LOX-1 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOX-1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LOX-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LOX-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LOX-1 and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LOX- 1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human LOX-1. You can calculate the concentration of Human LOX-1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4008187
• NCBI GeneID: 140914
• UniProt Accession #: O70156
• Molecular Weight: 41,890 Da
• NCBI Official Full Name: lectin-like oxidized low-density lipoprotein receptor
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: Olr1
• NCBI Official Synonym Symbols: LOX-1; Oldr1; Oldlr1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; Lectin-like oxidized low-density lipoprotein receptor-1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-like oxidized low-density lipoprotein receptor; lectin-type oxidized LDL receptor 1; ox-LDL receptor 1; oxidised low density lipoprotein (lectin-like) receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: Olr1
• UniProt Synonym Gene Names: Lox1; Oldlr1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1
• UniProt Entry Name: OLR1_RAT

NCBI Description

receptor for oxidized low-density lipoprotein that may be involved in pathogenesis of hypertension as well as atherosclerosis [RGD, Feb 2006]

Uniprot Description

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.