Human lipoprotein lipase (LPL) ELISA Kit | LPL elisa kit

Human lipoprotein lipase (LPL) ELISA Kit

Cat. Num. AAA267168

• Gene Names: LPL; LIPD; HDLCQ11
• Reactivity: Human
• Synonyms: lipoprotein lipase (LPL); Human lipoprotein lipase (LPL) ELISA Kit; LPL elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 475

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 200 ng/mL-3.12 ng/mL
• Sensitivity: Up to 0.6 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for LPL elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human LPL monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557727
• NCBI GeneID: 4023
• NCBI Accession #: NP_000228.1
• NCBI GenBank Nucleotide #: NM_000237.2
• Molecular Weight: 53,162 Da
• NCBI Official Full Name: lipoprotein lipase
• NCBI Official Synonym Full Names: lipoprotein lipase
• NCBI Official Symbol: LPL
• NCBI Official Synonym Symbols: LIPD; HDLCQ11
• NCBI Protein Information: lipoprotein lipase
• UniProt Protein Name: Lipoprotein lipase
• Protein Family: Lipoprotein
• UniProt Gene Name: LPL
• UniProt Synonym Gene Names: LIPD; LPL
• UniProt Entry Name: LIPL_HUMAN

NCBI Description

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Phospholipase; EC 3.1.1.34; Membrane protein, GPI anchor; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; plasma membrane; extracellular region

Molecular Function: heparin binding; triacylglycerol lipase activity; protein binding; lipoprotein lipase activity; apolipoprotein binding; phospholipase activity; triglyceride binding; receptor binding

Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; lipoprotein metabolic process; triacylglycerol biosynthetic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process

Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined