Human LIM Domain Binding Protein 1 (LDB1) ELISA Kit | LDB1 elisa kit

Human LIM Domain Binding Protein 1 (LDB1) ELISA Kit

Cat. Num. AAA455778

• Gene Names: LDB1; NLI; CLIM2; LDB-1; CLIM-2
• Reactivity: Human
• Synonyms: LIM Domain Binding Protein 1 (LDB1); Human LIM Domain Binding Protein 1 (LDB1) ELISA Kit; LDB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LDB1. No significant cross-reactivity or interference between LDB1 and analogues was observed.
• Sequence Length: 411

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LDB1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LDB1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDB1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LDB1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LDB1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LDB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LDB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LDB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 164663816
• NCBI GeneID: 8861
• NCBI Accession #: NP_001106878.1
• NCBI GenBank Nucleotide #: NM_001113407.2
• UniProt Accession #: Q86U70; O75479; O96010; Q1EQX1; Q9UGM4; B4DUC4
• Molecular Weight: 42,809 Da
• NCBI Official Full Name: LIM domain-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: LIM domain binding 1
• NCBI Official Symbol: LDB1
• NCBI Official Synonym Symbols: NLI; CLIM2; LDB-1; CLIM-2
• NCBI Protein Information: LIM domain-binding protein 1
• UniProt Protein Name: LIM domain-binding protein 1
• Protein Family: LIM domain-binding protein
• UniProt Gene Name: LDB1
• UniProt Synonym Gene Names: CLIM2; LDB-1; CLIM-2; hLdb1

Uniprot Description

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state ().