Human LIM Domain Binding Protein 1 (LDB1) ELISA Kit | LDB1 elisa kit

Human LIM Domain Binding Protein 1 (LDB1) ELISA Kit

Cat. Num. AAA2020109

• Gene Names: LDB1; NLI; CLIM2
• Reactivity: Human
• Synonyms: LIM Domain Binding Protein 1 (LDB1); Human LIM Domain Binding Protein 1 (LDB1) ELISA Kit; CLIM2; NLI; Carboxy Terminal LIM Domain Protein 2; LIM domain-binding factor CLIM2; Nuclear LIM interactor; LDB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LIM Domain Binding Protein 1 (LDB1).; No significant cross-reactivity or interference between LIM Domain Binding Protein 1 (LDB1) and analogues was observed.
• Sequence Length: 411

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDB1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM Domain Binding Protein 1 (LDB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM Domain Binding Protein 1 (LDB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM Domain Binding Protein 1 (LDB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM Domain Binding Protein 1 (LDB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LDB1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 164663816
• NCBI GeneID: 8861
• NCBI Accession #: NP_001106878.1
• NCBI GenBank Nucleotide #: NM_001113407.1
• UniProt Accession #: Q86U70; O75479; O96010; Q1EQX1; Q9UGM4; B4DUC4
• Molecular Weight: 42,809 Da
• NCBI Official Full Name: LIM domain-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: LIM domain binding 1
• NCBI Official Symbol: LDB1
• NCBI Official Synonym Symbols: NLI; CLIM2
• NCBI Protein Information: LIM domain-binding protein 1
• UniProt Protein Name: LIM domain-binding protein 1
• Protein Family: LIM domain-binding protein
• UniProt Gene Name: LDB1
• UniProt Synonym Gene Names: CLIM2; LDB-1; CLIM-2; hLdb1
• UniProt Entry Name: LDB1_HUMAN

Uniprot Description

LDB1: Binds to the LIM domain of a wide variety of LIM domain- containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. Belongs to the LDB family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 10q24-q25

Cellular Component: transcription factor complex; protein complex; nuclear chromatin; nucleus

Molecular Function: LIM domain binding; protein homodimerization activity; enzyme binding; protein self-association; chromatin binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of erythrocyte differentiation; Wnt receptor signaling pathway; positive regulation of cell adhesion; transcription, DNA-dependent; somatic stem cell maintenance; multicellular organismal development; gastrulation with mouth forming second; positive regulation of hemoglobin biosynthetic process; cellular component assembly; neuron differentiation; hair follicle development; regulation of transcription, DNA-dependent; cerebellar Purkinje cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of RNA elongation; anterior/posterior axis specification