Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leukocyte cell-derived chemotaxin-2 (LECT2) Standard Curve.)
Human Leukocyte Cell-Derived Chemotaxin-2 (LECT2) ELISA Kit | LECT2 elisa kit
Human Leukocyte Cell-Derived Chemotaxin-2 (LECT2) ELISA Kit
Gene Names
LECT2; chm2; chm-II
Reactivity
Human
Synonyms
Leukocyte Cell-Derived Chemotaxin-2 (LECT2); Human Leukocyte Cell-Derived Chemotaxin-2 (LECT2) ELISA Kit; LECT2; MGC126628; chm-II; chm2; chondromodulin-II; LECT2 elisa kit
Reactivity
Human
Specificity
Human Leukocyte cell-derived chemotaxin-2 (LECT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human LECT2. No significant cross-reactivity or interference between Human LECT2 and analogues was observed.
Sequence Length
151
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leukocyte cell-derived chemotaxin-2 (LECT2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leukocyte cell-derived chemotaxin-2 (LECT2) Standard Curve.)
Related Product Information for LECT2 elisa kit
Description: This Human Leukocyte cell-derived chemotaxin-2 (LECT2) ELISA Kit employs a two-site sandwich ELISA to quantitate LECT2 in samples. An antibody specific for LECT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LECT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LECT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LECT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Leukocyte cell-derived chemotaxin-2 is a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. Neutrophils migrate in response to increasing concentrations of chemotactic factors released from inflammatory sites. From the culture fluid of a human T-cell leukemia cell line (SKW-3), Yamagoe et al. (1996) isolated a 16-kD secreted protein with neutrophil chemotactic activity and designated it LECT2. They purified the protein and determined the amino acid sequence.
Background: Leukocyte cell-derived chemotaxin-2 is a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. Neutrophils migrate in response to increasing concentrations of chemotactic factors released from inflammatory sites. From the culture fluid of a human T-cell leukemia cell line (SKW-3), Yamagoe et al. (1996) isolated a 16-kD secreted protein with neutrophil chemotactic activity and designated it LECT2. They purified the protein and determined the amino acid sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
leukocyte cell-derived chemotaxin-2
NCBI Official Synonym Full Names
leukocyte cell derived chemotaxin 2
NCBI Official Symbol
LECT2
NCBI Official Synonym Symbols
chm2; chm-II
NCBI Protein Information
leukocyte cell-derived chemotaxin-2
UniProt Protein Name
Leukocyte cell-derived chemotaxin-2
Protein Family
UniProt Gene Name
LECT2
UniProt Synonym Gene Names
LECT-2; hLECT2
UniProt Entry Name
LECT2_HUMAN
NCBI Description
This gene encodes a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Uniprot Description
LECT2: Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. Belongs to the LECT2/MIM-1 family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space
Molecular Function: identical protein binding
Biological Process: skeletal development
Research Articles on LECT2
Similar Products
Product Notes
The Human LECT2 lect2 (Catalog #AAA9715499) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715499 ELISA Kit recognizes Human LECT2. It is sometimes possible for the material contained within the vial of "Leukocyte Cell-Derived Chemotaxin-2 (LECT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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