Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leukemia-associated protein 1 (DLEU1) Standard Curve.)
Human Leukemia-Associated Protein 1 (DLEU1) ELISA Kit | DLEU1 elisa kit
Human Leukemia-Associated Protein 1 (DLEU1) ELISA Kit
Reactivity
Human
Synonyms
Leukemia-Associated Protein 1 (DLEU1); Human Leukemia-Associated Protein 1 (DLEU1) ELISA Kit; DLEU1; RP11-175B12.1; BCMS; DLB1; DLEU2; LEU1; LEU2; MGC22430; NCRNA00021; XTP6; DLEU1 elisa kit
Reactivity
Human
Specificity
Human Leukemia-associated protein 1 (DLEU1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DLEU1. No significant cross-reactivity or interference between Human DLEU1 and analogues was observed.
Sequence Length
78
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leukemia-associated protein 1 (DLEU1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leukemia-associated protein 1 (DLEU1) Standard Curve.)
Related Product Information for DLEU1 elisa kit
Description: This Human Leukemia-associated protein 1 (DLEU1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLEU1 in samples. An antibody specific for DLEU1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLEU1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLEU1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLEU1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Deletion of chromosome 13q14 is the most frequent genetic aberration in B-cell chronic lymphocytic leukemia (CLL), found in more than 50% of cases, indicating that this region contains a gene(s) involved in the development of CLL.DLEU7, located adjacent to the consensus deleted region, and overlapping the 3' end of DLEU1 tail to tail. Human DLEU7 encodes a putative 221 amino acid protein, with significant conservation in rodents. Mutational and expression analysis in primary CLL samples failed to demonstrate any specific mutations in DLEU7, but no DLEU7 expression could be detected in CLL cells. Methylation of a CpG island in the promoter region of DLEU7 was further analyzed as a possible mechanism for the absence of DLEU7 expression, and the promoter was found to be methylated in the majority of the CLL samples investigated.
Background: Deletion of chromosome 13q14 is the most frequent genetic aberration in B-cell chronic lymphocytic leukemia (CLL), found in more than 50% of cases, indicating that this region contains a gene(s) involved in the development of CLL.DLEU7, located adjacent to the consensus deleted region, and overlapping the 3' end of DLEU1 tail to tail. Human DLEU7 encodes a putative 221 amino acid protein, with significant conservation in rodents. Mutational and expression analysis in primary CLL samples failed to demonstrate any specific mutations in DLEU7, but no DLEU7 expression could be detected in CLL cells. Methylation of a CpG island in the promoter region of DLEU7 was further analyzed as a possible mechanism for the absence of DLEU7 expression, and the promoter was found to be methylated in the majority of the CLL samples investigated.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Leukemia-associated protein 1
UniProt Protein Name
Leukemia-associated protein 1
Protein Family
UniProt Gene Name
DLEU1
UniProt Synonym Gene Names
LEU1; XTP6
UniProt Entry Name
LEU1_HUMAN
Uniprot Description
DLEU1: May act as a tumor suppressor
Protein type: Tumor suppressor
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Product Notes
The Human DLEU1 dleu1 (Catalog #AAA9715695) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715695 ELISA Kit recognizes Human DLEU1. It is sometimes possible for the material contained within the vial of "Leukemia-Associated Protein 1 (DLEU1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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