Human Leucine Rich Repeat Containing Protein 4B (LRRC4B) ELISA Kit | LRRC4B elisa kit

Human Leucine Rich Repeat Containing Protein 4B (LRRC4B) ELISA Kit

Cat. Num. AAA456335

• Gene Names: LRRC4B; HSM; LRIG4
• Reactivity: Human
• Synonyms: Leucine Rich Repeat Containing Protein 4B (LRRC4B); Human Leucine Rich Repeat Containing Protein 4B (LRRC4B) ELISA Kit; LRRC4B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LRRC4B. No significant cross-reactivity or interference between LRRC4B and analogues was observed.
• Sequence Length: 713

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.133ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LRRC4B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LRRC4B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LRRC4B elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LRRC4B in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LRRC4B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LRRC4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LRRC4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LRRC4B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 122937309
• NCBI GeneID: 94030
• NCBI Accession #: NP_001073926.1
• NCBI GenBank Nucleotide #: NM_001080457.1
• UniProt Accession #: Q9NT99; Q3ZCQ4; Q58F20
• Molecular Weight: 76,434 Da
• NCBI Official Full Name: leucine-rich repeat-containing protein 4B
• NCBI Official Synonym Full Names: leucine rich repeat containing 4B
• NCBI Official Symbol: LRRC4B
• NCBI Official Synonym Symbols: HSM; LRIG4
• NCBI Protein Information: leucine-rich repeat-containing protein 4B
• UniProt Protein Name: Leucine-rich repeat-containing protein 4B
• Protein Family: Leucine-rich repeat-containing protein
• UniProt Gene Name: LRRC4B
• UniProt Synonym Gene Names: LRIG4; NGL-3

Uniprot Description

Synaptic adhesion protein. Regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and PTPRF regulates the formation of excitatory synapses in a bidirectional manner ().