Human Leucine-Rich Repeat And Calponin Homology Domain-Containing Protein 4 (LRCH4) ELISA Kit | LRCH4 elisa kit

Human Leucine-Rich Repeat And Calponin Homology Domain-Containing Protein 4 (LRCH4) ELISA Kit

Cat. Num. AAA456894

• Gene Names: LRCH4; LRN; LRRN1; LRRN4; PP14183
• Reactivity: Human
• Synonyms: Leucine-Rich Repeat And Calponin Homology Domain-Containing Protein 4 (LRCH4); Human Leucine-Rich Repeat And Calponin Homology Domain-Containing Protein 4 (LRCH4) ELISA Kit; LRN; LRRN4; SAP25; leucine rich repeat protein 4; neuronal; LRCH4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LRCH4. No significant cross-reactivity or interference between LRCH4 and analogues was observed.
• Sequence Length: 652

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: <27pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LRCH4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LRCH4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LRCH4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LRCH4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LRCH4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LRCH4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LRCH4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LRCH4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 584277014
• NCBI GeneID: 4034
• NCBI Accession #: NP_001276863.1
• NCBI GenBank Nucleotide #: NM_001289934.1
• UniProt Accession #: O75427; Q8WV85; Q96ID0; A4D2D5
• Molecular Weight: 73,450 Da
• NCBI Official Full Name: leucine-rich repeat and calponin homology domain-containing protein 4 isoform 2
• NCBI Official Synonym Full Names: leucine rich repeats and calponin homology domain containing 4
• NCBI Official Symbol: LRCH4
• NCBI Official Synonym Symbols: LRN; LRRN1; LRRN4; PP14183
• NCBI Protein Information: leucine-rich repeat and calponin homology domain-containing protein 4
• UniProt Protein Name: Leucine-rich repeat and calponin homology domain-containing protein 4
• Protein Family: Leucine-rich repeat and calponin homology domain-containing protein
• UniProt Gene Name: LRCH4
• UniProt Synonym Gene Names: LRN; LRRN1; LRRN4; Leucine-rich neuronal protein

NCBI Description

This gene encodes a protein that contains leucine-rich repeats (LRR) at its amino terminus and that is known to be involved in ligand binding. The carboxyl terminus may act as a membrane anchor. Identified structural elements suggest that the encoded protein resembles a receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

LRCH4:

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: basolateral plasma membrane; cytoplasm; PML body

Molecular Function: protein binding

Biological Process: maintenance of epithelial cell polarity; nervous system development; signal transduction