Typical Testing Data/Standard Curve (for reference only)
Human Leptin Receptor ELISA Kit | LEPR elisa kit
Leptin Receptor ELISA
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-KIM-1 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-KIM-1 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the KIM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of KIM-1 can be calculated!!Background/Introduction: KIM1, also known as Hepatitis A virus cellular receptor 1, is a protein that in rats is encoded by the HAVCR1 gene, which maps to 5q33.2. It is a major cause of orally transmitted acute hepatitis, infects primate cells, but not dog or rat cells, after binding to the HAV cellular receptor (HAVCR). Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Khademi et al. (2004) found that differential expression of TIMs by Th1 and Th2 cells may be implicated in different phases of an autoimmune disease.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
Uniprot Description
LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 1p31
Cellular Component: extracellular region; integral to membrane; plasma membrane; receptor complex
Molecular Function: identical protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; transmembrane receptor activity
Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of gluconeogenesis; multicellular organismal development; negative regulation of hydrolase activity; cytokine and chemokine mediated signaling pathway; energy reserve metabolic process
Disease: Leptin Receptor Deficiency
Research Articles on LEPR
Similar Products
Product Notes
The Human LEPR lepr (Catalog #AAA494793) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494793 ELISA Kit recognizes Human LEPR. It is sometimes possible for the material contained within the vial of "Leptin Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
