Rabbit anti-Human DDX21 Polyclonal Antibody | anti-DDX21 antibody

DDX21, NT (DDX21, Nucleolar RNA helicase 2, DEAD box protein 21, Gu-alpha, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/Gu) (AP)

Cat. Num. AAA6291387

• Gene Names: DDX21; GUA; GURDB; RH-II/GU; RH-II/GuA; DKFZp686F21172
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX21; Polyclonal Antibody; NT (DDX21; Nucleolar RNA helicase 2; DEAD box protein 21; Gu-alpha; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu) (AP); RH II/Gu; anti-DDX21 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DDX21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DDX21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the N-terminal region of human DDX21.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DDX21 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the DDX21 antibody detected the DDX21 protein (arrow).)

Related Product Information for anti-DDX21 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.

Product Categories/Family for anti-DDX21 antibody

Antibodies; DEAD Box Proteins

References

Holmstrom, T.H., et al. J. Biol. Chem. 283(11):7046-7053(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 50659095
• NCBI GeneID: 9188
• NCBI Accession #: NP_004719.2
• NCBI GenBank Nucleotide #: NM_004728.2
• UniProt Accession #: Q9NR30; Q13436; Q5VX41; Q68D35; B2RDL0; Q3SWU7; Q8NI92
• Molecular Weight: 87,344 Da
• NCBI Official Full Name: nucleolar RNA helicase 2
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• NCBI Official Symbol: DDX21
• NCBI Official Synonym Symbols: GUA; GURDB; RH-II/GU; RH-II/GuA; DKFZp686F21172
• NCBI Protein Information: nucleolar RNA helicase 2; RH II/Gu; gu-alpha; Gu protein; OTTHUMP00000019713; DEAD box protein 21; RNA helicase II/Gu alpha; nucleolar RNA helicase Gu; nucleolar RNA helicase II; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
• UniProt Protein Name: Nucleolar RNA helicase 2
• Protein Family: Nucleolar RNA helicase
• UniProt Gene Name: DDX21
• UniProt Entry Name: DDX21_HUMAN

Uniprot Description

DDX21: a protein of the DEAD box helicase family. Unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: EC 3.6.4.13; RNA processing; Helicase; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: membrane; nucleolus; nucleus

Molecular Function: double-stranded RNA binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: osteoblast differentiation; response to exogenous dsRNA; metabolic process; response to virus